Molecular and Cellular Analysis of Lipogems-Processed Lipoaspirates for Evaluating the Efficacy of Treatments in Regenerative Medicine

Autologous adipose tissue is a readily available source of mesenchymal stromal cells (MSCs) for regenerative applications. Here, human lipoaspirates from eight donors processed with the Lipogems system were characterized by flow cytometry (MSC/pericyte markers and inflammatory markers), immunocytochemistry (NANOG, OCT3/4, SOX2), and functional assays assessing proliferation, senescence, apoptosis, multilineage differentiation, IDO activity, and ROS levels. Lipogems-derived cultures fulfilled MSC immunophenotypic criteria while showing marked inter-donor variability in MSC/pericyte proportions, stemness-marker expression, differentiation bias, inflammatory profile, and oxidative status. In contrast, senescence and apoptosis were comparable across samples. Clinical evaluation, based on surgeon-assessed outcomes after autologous treatment, scored on a 1–10 scale, was consistently high across donors (7–10). Overall, Lipogems-processed lipoaspirates harbor a heterogeneous but functionally competent stromal compartment; these data support a sample-specific quality framework (composition, differentiation tendency, inflammation/ROS, IDO responsiveness) to improve reproducibility and optimization of regenerative protocols.