The development of in vitro embryo production (IVEP) techniques in Felis catus is a fittingmodel with potential application to the conservation of endangered felid species. To improvethe quality of IVEP techniques an appropriate balance of pro- and antioxidants shouldbe provided. Under in vitro conditions, high levels of superoxide dismutase (SOD), glutathioneperoxidase (GPx) and catalase (CAT) mRNA provide a defence mechanism against oxidativestress for embryos. In order to improve the development of cat oocytes, the effects of SOD andCAT supplemented to in vitro maturation (IVM) medium and of GPx supplemented to in vitrofertilization (IVF) medium on development and embryo production in vitro were evaluated.Data showed an increase of 70 and 77 % of cleaved embryo and blastocyst formation, respectively,in the experiment with SOD and CAT addition to IVM medium; in the experimentwith GPx addition to IVF medium the number of cleaved embryos doubled and the numberof embryos increased by 96 %. Therefore, our results were positive and encourage us to continuestudies on cat oocytes evaluating the effects of various dosages and combination of antioxidants.
Antioxidant supplementation to medium for in vitro embryo production in Felis catus
C. Del Prete;
2019-01-01
Abstract
The development of in vitro embryo production (IVEP) techniques in Felis catus is a fittingmodel with potential application to the conservation of endangered felid species. To improvethe quality of IVEP techniques an appropriate balance of pro- and antioxidants shouldbe provided. Under in vitro conditions, high levels of superoxide dismutase (SOD), glutathioneperoxidase (GPx) and catalase (CAT) mRNA provide a defence mechanism against oxidativestress for embryos. In order to improve the development of cat oocytes, the effects of SOD andCAT supplemented to in vitro maturation (IVM) medium and of GPx supplemented to in vitrofertilization (IVF) medium on development and embryo production in vitro were evaluated.Data showed an increase of 70 and 77 % of cleaved embryo and blastocyst formation, respectively,in the experiment with SOD and CAT addition to IVM medium; in the experimentwith GPx addition to IVF medium the number of cleaved embryos doubled and the numberof embryos increased by 96 %. Therefore, our results were positive and encourage us to continuestudies on cat oocytes evaluating the effects of various dosages and combination of antioxidants.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
