Animals carrying translocations may present alterations in the meiotic process due to the presence ofthe translocated chromosomes. Several chromosomal abnormalities have been reported to date inriver buffalo, analysing only the somatic cells, with no evidence about both the fertility andinformation about germ cells affected by the aberration. The aim of this work was to study, for thefirst time, animal fertility by meiotic segregation patterns in a river buffalo bull, carrier of de novotranslocation t (1p;18). A triple-colour fluorescent in situ hybridization (FISH) in sperms wasperformed on chromosomes involved in the translocation (BBU1 and BBU18) using three differentpools of specific bovine BAC probes mapping in BBU1q, BBU1p and BBU18 (homologous to BTA1,BTA27 and BTA18, respectively). At the moment, the meiotic segregation pattern was examined in2500 sperms of the carrier and 2500 sperms of another river buffalo bull, used as control. Of all thegametes analyzed, the frequencies of normal and chromosomally balanced sperms (alternate group)were 25.63 % and 11.11 % respectively (total of 36.74 %) in the carrier; while in the control were97.16 % and 0.69 % (total of 97.85 %), respectively. The frequencies of each sperm product resultingfrom adjacent I, adjacent II and 3:1 segregation were 18.2 %, 1.5%and 36%in the carrier, respectively;while in the control were 0.4 % in adjacent I and 1.6 % in 3:1 segregation. These data have shown asignificant difference between a bull carrier the translocation and the normal bull, suggesting theimportance of this analysis to evaluate the fertility in reproducers. In conclusion, Sperm Fish analysishas given useful data about the incidence of chromosomally balanced, unbalanced and aneuploidgametes, but needs to be completed by the study of other kinds of chromosomal rearrangements onboth total and living sperms to improve our knowledge about the effects of chromosomal aberrationson germ cells.
Sperm FISH analysis of meiotic segregation in a river buffalo bull carrier of t(1p;18)
Giannico, F;
2016-01-01
Abstract
Animals carrying translocations may present alterations in the meiotic process due to the presence ofthe translocated chromosomes. Several chromosomal abnormalities have been reported to date inriver buffalo, analysing only the somatic cells, with no evidence about both the fertility andinformation about germ cells affected by the aberration. The aim of this work was to study, for thefirst time, animal fertility by meiotic segregation patterns in a river buffalo bull, carrier of de novotranslocation t (1p;18). A triple-colour fluorescent in situ hybridization (FISH) in sperms wasperformed on chromosomes involved in the translocation (BBU1 and BBU18) using three differentpools of specific bovine BAC probes mapping in BBU1q, BBU1p and BBU18 (homologous to BTA1,BTA27 and BTA18, respectively). At the moment, the meiotic segregation pattern was examined in2500 sperms of the carrier and 2500 sperms of another river buffalo bull, used as control. Of all thegametes analyzed, the frequencies of normal and chromosomally balanced sperms (alternate group)were 25.63 % and 11.11 % respectively (total of 36.74 %) in the carrier; while in the control were97.16 % and 0.69 % (total of 97.85 %), respectively. The frequencies of each sperm product resultingfrom adjacent I, adjacent II and 3:1 segregation were 18.2 %, 1.5%and 36%in the carrier, respectively;while in the control were 0.4 % in adjacent I and 1.6 % in 3:1 segregation. These data have shown asignificant difference between a bull carrier the translocation and the normal bull, suggesting theimportance of this analysis to evaluate the fertility in reproducers. In conclusion, Sperm Fish analysishas given useful data about the incidence of chromosomally balanced, unbalanced and aneuploidgametes, but needs to be completed by the study of other kinds of chromosomal rearrangements onboth total and living sperms to improve our knowledge about the effects of chromosomal aberrationson germ cells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
