Proteomics and Epigenetic Mechanisms in Stem Cells

Epigenetic mechanisms orchestrate inheritable concerted networks essential for chromatin remodeling. Molecularinterplays include post-translational modifications to histones, DNA methylation, activity of small non coding RNAs,govern activation and silencing of gene expression and define the molecular basis of pluripotency, reprogramming, earlyhuman development and differentiation.The implications of epigenetic regulation in maintaining stem cell fate determination are well known. Thus: (i) embryonicstem cells (ESCs) seem to employ selected histone modification mechanisms for maintaining pluripotency and for the activationof multipotency programs; (ii) induced pluripotent stem cells, while recapitulating the overall features of theESCs epigenome, express differences in DNA methylation; (iii) the lineage-restricted process of adult mesenchymal stemcell differentiation involves epigenetic regulation and consists of a unique pattern of DNA methylation and histone modifications;(iv) the epigenetic profiles of adult stem cells correlate with a more restricted differentiation potential as comparedto ESCs.This review will discuss the role of epigenetic regulation in pluripotency, stemness and cell fate specification, taking advantageof recent discoveries showing that mass spectrometry and proteomics are become indispensable tools in epigeneticresearch.