Stem cells are a promising tool for bone tissue regeneration. Dental pulp stem cells (DPSCs) can be easilyobtained even in human young adults. In this study we investigated the capability of DPSCs, to expressthe osteoblastic phenotype when cultured with osteogenic medium. DPSCs isolated from the dental pulpof impacted third molar teeth were cultured with appropriate medium to induce osteoblast differentiation.UsingWestern-Blot, RI-PCR and microarray analysis, we studied the expression of osteoblastic parameter,and by Von Kossa staining we evaluated the production of mineralized matrix nodules. The results werecompared with controls represented by undifferentiated DPSCs. DPSCs, differentiated into osteoblastlikecells, express large amount of alkaline phosphatase (ALP), collagen I (Cotl f), osteopontin (OPN) andosteocalcin (OCl$, all these parameters characterizing the osteoblastic phenotype. Differentiated DPSCsexpress Runx2 and JunB, a member of the AP-l complex; both the transcription factors are associatedwith osteoblast differentiation and skeletal morphogenesis. Moreover, DPSCs express insulin growthfactor-binding protein 5 (IGFBP-S), one of the regulating proteins of IGFs function. Finally DPSCs canform mineralized matrix nodules that are a feature exclusive to osteoblasts. DPSCs could represent apotential source ofosteoblasts to be used for bone regeneration.
Osteogenic properties of human dental pulp stem cells
BALLINI A;
2010-01-01
Abstract
Stem cells are a promising tool for bone tissue regeneration. Dental pulp stem cells (DPSCs) can be easilyobtained even in human young adults. In this study we investigated the capability of DPSCs, to expressthe osteoblastic phenotype when cultured with osteogenic medium. DPSCs isolated from the dental pulpof impacted third molar teeth were cultured with appropriate medium to induce osteoblast differentiation.UsingWestern-Blot, RI-PCR and microarray analysis, we studied the expression of osteoblastic parameter,and by Von Kossa staining we evaluated the production of mineralized matrix nodules. The results werecompared with controls represented by undifferentiated DPSCs. DPSCs, differentiated into osteoblastlikecells, express large amount of alkaline phosphatase (ALP), collagen I (Cotl f), osteopontin (OPN) andosteocalcin (OCl$, all these parameters characterizing the osteoblastic phenotype. Differentiated DPSCsexpress Runx2 and JunB, a member of the AP-l complex; both the transcription factors are associatedwith osteoblast differentiation and skeletal morphogenesis. Moreover, DPSCs express insulin growthfactor-binding protein 5 (IGFBP-S), one of the regulating proteins of IGFs function. Finally DPSCs canform mineralized matrix nodules that are a feature exclusive to osteoblasts. DPSCs could represent apotential source ofosteoblasts to be used for bone regeneration.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
