An analytical method for the determination of eight pharmaceutical compounds in waters has been developed and validated. Target compounds included hormones, non-steroidal anti-inflammatory drugs, antibiotics, β-blockers and psychiatric drugs.The analytical determination has been achieved through off-line solid phase extraction and analysis in liquid chromatography coupled with mass spectrometry.For the determination of the hormones, samples were also derivatized with 1,2-dimethylimidazole-4-sulfonyl-chloride. The limits of detection of the drugs varied between 0.01 and 30 ng/L, according to the limits indicated by the European Commission. For each compound, the six-point calibration curves showed excellent linearity (r2 ≥ 0.995) and the inter-session repeatability for all compounds ranged from 1 to 10%. This method was also applied for the determination of the target pharmaceuticals in the influent and the effluent of a wastewater treatment plant in order to assess their presence and their removal inside the plant. Every compound, except for 17-α-ethinylestradiol, has been found in the influentof the wastewater treatment plant and none of them, except for acetaminophen, were removed within the plant.
Development of an automated LC-MS/MS method for the determination of eight pharmaceutical compounds in wastewater
Dugheri, Stefano
;
2018-01-01
Abstract
An analytical method for the determination of eight pharmaceutical compounds in waters has been developed and validated. Target compounds included hormones, non-steroidal anti-inflammatory drugs, antibiotics, β-blockers and psychiatric drugs.The analytical determination has been achieved through off-line solid phase extraction and analysis in liquid chromatography coupled with mass spectrometry.For the determination of the hormones, samples were also derivatized with 1,2-dimethylimidazole-4-sulfonyl-chloride. The limits of detection of the drugs varied between 0.01 and 30 ng/L, according to the limits indicated by the European Commission. For each compound, the six-point calibration curves showed excellent linearity (r2 ≥ 0.995) and the inter-session repeatability for all compounds ranged from 1 to 10%. This method was also applied for the determination of the target pharmaceuticals in the influent and the effluent of a wastewater treatment plant in order to assess their presence and their removal inside the plant. Every compound, except for 17-α-ethinylestradiol, has been found in the influentof the wastewater treatment plant and none of them, except for acetaminophen, were removed within the plant.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
