Characterization of adipose-derived stem cells freshly isolated from liposuction aspirates performed with Prolipostem®

Background: Lipofilling is a cosmetic surgical procedure that consists in the withdrawal of a small quantity of fattissue from a suitable anatomic area and the reimplantation of this tissue in another corporeal district in the sameindividual, so as to obtain a filling effect. Recently, adipose-derived stem cells (ADSCs) have been isolated in theaspirates. These are pluripotent mesenchymal stem cells that are able to differentiate in mature adipose cells orother adult mesenchymal cells after paracrine or autocrine hormonal stimulations, thus favoring a longer survivalof the implanted tissues.Methods: In this article, we have defined a new method for liposuction (Prolipostem®), where the ADSCs are recoveredand mixed with the suctioned adipose tissue derived from the abdominal fat, before the reimplantation.Results: We have demonstrated by immunocytochemistry the presence of ADSCs in the adipose tissue taken withProlipostem® from the abdominal fat and the ability of these ADSCs to differentiate in mature adipose cells in vitro.Conclusions: The possibility to enrich the tissue to be implanted with ADSCs would assure a longer survival of the cellsimplanted and a regeneration of the host tissue thanks to growth and angiogenic stimuli induced by the ADSCs.