Aim of work. The carotid artery stenting (CAS) is the treatment of extracranial lesions as the ischemic stroke and TIA (transient ischemic attack). The measurements of circulating inflammatory and anti- inflammatory markers, including interleukin-6 (IL-6) (Harris et al, 1999; Ridker et al, 2000) and IL-10, carry important information as predictors of future vascular events in CAS patients. We have studied 17 patients before and after CAS(1 week, 1month and 1 year)to find out potential variations of IL-6 and IL-10 in the plasma.Materials and Methods. From February to May 2009, have been recruited the patients of both sexes, symptomatic and asymptomatic, affected by carotid stenosis higher than 70%, candidate to CAS intervention. The patients have been subject to four venous blood withdrawal, collected in sodium ethylenediamine tetraacetate tubes, carried out with the following timing: before surgery (Baseline), one week (T1), one month (T2) and one year after stenting(T3). The collected blood samples were stored at -80°C. Subsequently, IL-6(pg/mL) and IL-10(pg/mL) were analyzed(Randox EVIDENCE Biochip Array). The data were expressed as media with 95% CI. Friedman test for repeated measures with Dunn’s multiple comparison test were used to analyze results.Results. The mean age(mean±SD) of the patients was 73.5(7.1) years. The IL-6 increased significantly at T1 compared to baseline(5.21±2.73 vs 2.30±1.06 respectively, p<0.01) and subsequently at T2 and T3 the plasma concentration decreased(p<0.0001). The IL-10 increased significantly at T2 respect to baseline(1.63±0.83 vs 0.81±0.22 respectively, p<0.05) and at to T3 the concentration decreased but remained at higher values compared to baseline(p=0.02).Discussion and Conclusion. This study showed that IL-6 and IL-10 are related with inflammatory activity of the plaque. Different study showed that IL-6 expression level is significantly elevated in carotid lesions collected from patients who had suffered from a recent stroke (Peeters et al, 2009). Our results, suggested the possibility that IL-6 and IL-10 could be released into the plaque and transferred in the blood circulation. The association between IL-6 and IL-10 levels suggests that exist a relation among inflammatory and anti-inflammatory balance demonstrated to the specular variation of the cytokines plasma concentration. In conclusion, IL-6 and IL-10 could be used in thefollow-up of patients after CAS.
IL6 and IL10 activity in patients with carotid artery stenting (CAS)
RANIERI, DANILO;
2011-01-01
Abstract
Aim of work. The carotid artery stenting (CAS) is the treatment of extracranial lesions as the ischemic stroke and TIA (transient ischemic attack). The measurements of circulating inflammatory and anti- inflammatory markers, including interleukin-6 (IL-6) (Harris et al, 1999; Ridker et al, 2000) and IL-10, carry important information as predictors of future vascular events in CAS patients. We have studied 17 patients before and after CAS(1 week, 1month and 1 year)to find out potential variations of IL-6 and IL-10 in the plasma.Materials and Methods. From February to May 2009, have been recruited the patients of both sexes, symptomatic and asymptomatic, affected by carotid stenosis higher than 70%, candidate to CAS intervention. The patients have been subject to four venous blood withdrawal, collected in sodium ethylenediamine tetraacetate tubes, carried out with the following timing: before surgery (Baseline), one week (T1), one month (T2) and one year after stenting(T3). The collected blood samples were stored at -80°C. Subsequently, IL-6(pg/mL) and IL-10(pg/mL) were analyzed(Randox EVIDENCE Biochip Array). The data were expressed as media with 95% CI. Friedman test for repeated measures with Dunn’s multiple comparison test were used to analyze results.Results. The mean age(mean±SD) of the patients was 73.5(7.1) years. The IL-6 increased significantly at T1 compared to baseline(5.21±2.73 vs 2.30±1.06 respectively, p<0.01) and subsequently at T2 and T3 the plasma concentration decreased(p<0.0001). The IL-10 increased significantly at T2 respect to baseline(1.63±0.83 vs 0.81±0.22 respectively, p<0.05) and at to T3 the concentration decreased but remained at higher values compared to baseline(p=0.02).Discussion and Conclusion. This study showed that IL-6 and IL-10 are related with inflammatory activity of the plaque. Different study showed that IL-6 expression level is significantly elevated in carotid lesions collected from patients who had suffered from a recent stroke (Peeters et al, 2009). Our results, suggested the possibility that IL-6 and IL-10 could be released into the plaque and transferred in the blood circulation. The association between IL-6 and IL-10 levels suggests that exist a relation among inflammatory and anti-inflammatory balance demonstrated to the specular variation of the cytokines plasma concentration. In conclusion, IL-6 and IL-10 could be used in thefollow-up of patients after CAS.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
