A micromethod that allows polymerase chain reaction (PCR) amplification of proto-oncogene sequences from DNA extracted from thyroid FNA cytologic smears has been set up. A point mutation at codon 12 of H-ras gene was identified in one case, cytologically classified as follicular neoplasia and histologically diagnosed as follicular carcinoma. The method described may be useful for the preoperative identification of cancer-associated oncogenic alterations in those nodules that cannot be diagnosed at conventional light microscopy evaluation
Detection of ras mutation in fine needle aspiration cytologic specimens from neoplastic follicular nodules of the thyroid
SCIACCHITANO, Salvatore;
1992-01-01
Abstract
A micromethod that allows polymerase chain reaction (PCR) amplification of proto-oncogene sequences from DNA extracted from thyroid FNA cytologic smears has been set up. A point mutation at codon 12 of H-ras gene was identified in one case, cytologically classified as follicular neoplasia and histologically diagnosed as follicular carcinoma. The method described may be useful for the preoperative identification of cancer-associated oncogenic alterations in those nodules that cannot be diagnosed at conventional light microscopy evaluationFile in questo prodotto:
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